Apparatus and method for fiducial marker alignment in electron tomography

An apparatus for fiducial marker alignment, the apparatus comprising a processor and a memory storing computer instructions that, when executed by the processor, cause the apparatus to:

• align positions of fiducial markers on two or more micrographs, wherein each micrograph is formed of a corresponding point set,
• create a first set of matched fiducial markers and a first set of unmatched fiducial markers for each micrograph of the two or more micrographs;
• and
• transform the first set of unmatched fiducial markers of a first micrograph into a transformed point sets and match (1) the transformed point set to (2) the first set of unmatched fiducial markers of a second micrograph, resulting in a second set of matched fiducial markers for the first micrograph,
• wherein the second set of matched fiducial markers for the first micrograph results in improved alignment of a number of fiducial markers, in addition to the first set of matched fiducial markers, for the first micrograph.