US 11,307,398 B2Granted

Method for generating and analyzing an overview contrast image

AssigneeCarl Zeiss Microscopy GmbH

InventorsDaniel Haase, Thomas Ohrt, Markus Sticker

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Abstract

A method for generating and analyzing an overview contrast image of a specimen carrier and/or of specimens situated on a specimen carrier. A specimen carrier arranged at least partially in the focus of a detection optical unit is illuminated in transmitted light using a two-dimensional, array-like illumination pattern. At least two overview raw images are detected using different illuminations of the specimen carrier, and, according to information to be extracted from the overview contrast image, a combination algorithm is selected by means of which the at least two overview raw images are combined to form the overview contrast image. According to information to be extracted from the overview contrast image, an image evaluation algorithm is selected by means of which the information is extracted.

Claims20

Dependent claims

A method for producing and analyzing an overview contrast image of a sample carrier or of samples arranged on said sample carrier or of both the sample carrier and the samples arranged on said sample carrier, comprising:

illuminating the sample carrier, which is arranged at least partially in a focus of a detection optical unit, in transmitted light with a two-dimensional, array-type illumination pattern,
detecting at least two overview raw images with different illuminations of the sample carrier,
choosing a calculation algorithm that is used to calculate the overview contrast image with an increased contrast or an improved signal-to-noise ratio from the at least two overview raw images in dependence on information that is to be extracted from the overview contrast image, and
choosing an image evaluation algorithm that is used to extract the information from the overview contrast image in dependence on information that is to be extracted from the overview contrast image.

1 more element

Dependencies are inferred from the claim text.

Facts

Application: 16/618,773Filed 15 May 2018
Priority: DE 102017111718.030 May 2017
Published: US 2020/0088984 A119 Mar 2020
Granted: 19 Apr 2022
Term ends: Not estimatedThe source records don’t support an estimate.

Classification4 codes

G02B 21/125
Affording bright-field illumination: affording both dark- and bright-field illumination
G02B 21/086
Condensers: for transillumination only
G02B 21/367
Control or image processing arrangements for digital or video microscopes: providing an output produced by processing a plurality of individual…
G02B
Optical elements, systems or apparatus

Family

US 2020/0088984 A1Pre-grant publication · published 19 Mar 2020
US 11,307,398 B2This patent

From the US filing history. Foreign family members aren’t listed.

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