Functionalized metal-labeled beads for mass cytometry
AssigneeDeutsches Rheuma-Forschungszentrum Berlin
InventorsHenrik Mei, Axel Ronald Schulz, Lisa Budzinski
Life of this patent
Estimating the term…
Abstract
Beads for use as a control, calibration and/or quantification probe in a mass cytometry assay, wherein the beads are labeled with a heavy metal selected from the group comprising osmium or ruthenium. Also disclosed are beads labeled with a heavy metal exhibiting a surface functionalization that allows for the binding of an affinity reagent, such as a metal-conjugated antibody. Methods are described for the labeling of the beads and usage of the beads for quantification of cell surface receptors or for a compensation of channel crosstalk in mass cytometry assays.
Claims9
Method of analyzing beads as control, calibration and/or quantification probes in a mass cytometry assay, comprising the steps of
- a. providing a mass cytometer
- b. providing cells to be probed in said mass cytometer
- c. providing beads, the beads are labeled with a heavy metal selected from the group consisting of osmium and ruthenium, wherein the beads are synthetic polymeric beads and have been labelled with osmium by incubating the beads with an osmium tetroxide dilution or have been labeled with ruthenium by incubation the beads with a ruthenium tetroxide dilution,
- d. performing a mass cytometry assay including the steps of incubating the cells with one or more mass tagged affinity reagents, wherein the one or more mass tagged affinity reagents are metal conjugated antibodies configured for a specific binding to cellular target molecules of said cells such that a quantity of mass tagged affinity reagents allows for a determination of the presence of the cellular target molecules on said cells, and conducting an elemental analysis of said cells and beads using the mass cytometer,
- wherein said beads are analyzed as a control, calibration and/or quantification probe in said mass cytometry assay.
Dependencies are inferred from the claim text.