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US 11,307,205 B2Granted
Non-radioactive cytotoxicity assays
AssigneeUniversity of Southern California
InventorPreet M. Chaudhary
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Abstract
Described herein are methods for assessing cytotoxicity of an agent. The methods include providing a target cell that has been engineered to express intracellularly a reporter that is not expressed endogenously by the target cell, exposing the target cell to an agent capable of modulating cytotoxicity and assaying the activity of the reporter, wherein a change in reporter activity relative to a reference value is indicative of the agent being able to modulate cytotoxicity of the target cell.
Claims32
A single-step method for assessing cytotoxicity of an agent comprising:
- exposing a target cell that has been engineered to express intracellularly a thermostable non-secretory luciferase reporter to an agent capable of modulating cytotoxicity;
- and
- assaying the activity of the reporter to obtain the reporter activity,
- wherein the reporter is not expressed endogenously by the target cell or is expressed endogenously by the target cell at a level lower than what is achieved by engineered expression;
- and
- wherein a change in the reporter activity relative to a reference value is indicative of the agent being able to modulate cytotoxicity of the target cell;
- and
- wherein the reporter activity is assayed in the cell media containing the target cells.
5 more elements
Dependencies are inferred from the claim text.