Back to results
US 11,307,202 B1Granted
Antibody binding detection method for detecting MERS-CoV
AssigneeKing Abdulaziz University
InventorsAyman Talaat Abbas Abdelhadi, Esam Ibraheem Ahmed Azhar, Sherif Aly Abdelkhalek Elkafrawy, Sayed Sartaj Sohrab
Life of this patent
Estimating the term…
Abstract
A method for detecting MERS-CoV at high sensitivity and specificity using IgY antibodies that bind to MERS-CoV N protein, its fragments and domains. Isolated or purified IgY monospecific antibodies to MERS-CoV N protein.
Claims14
An antibody binding method for detecting MERS-CoV in Camelidae, comprising
- obtaining a nasopharyngeal swab sample from a subject,
- contacting the nasopharyngeal swab sample with a monospecific IgY antibody that recognizes MERS-CoV nucleocapsid protein (“N protein”), wherein the contacting includes contacting the sample with a calcium alginate swab to which the monospecific IgY antibody is bound, then removing a remaining unbound portion of the nasopharyngeal swab sample, and
- detecting complex formation or binding of the IgY antibody to the MERS-CoV,
- wherein complex formation or binding is detected with an ELISA sandwich assay, wherein the IgY antibody that recognizes MERS-CoV N protein is a capturing antibody bound to a substrate, wherein the capturing antibody bound to the substrate is contacted with the sample, and wherein complex formation is measured by the binding of a tagged secondary antibody that recognizes MERS-CoV,
- wherein the substrate is a microtiter plate well or a chromatography bead,
- wherein the tagged secondary antibody is bioconjugated to an enzyme.
3 more elements
Dependencies are inferred from the claim text.