Systems and methods for detection of chemtiluminescent reactions

A method for analyzing a sample, the method comprising:

• (a) obtaining a sample comprising 1,5-anhydroglucitol and a first analyte;
• (b) adding glucose oxidase to the sample, wherein the glucose oxidase causes a first chemiluminescent reaction with the sample;
• (c) measuring photons from a first light response resulting from the first chemiluminescent reaction;
• (d) adding pyranose oxidase to the sample, wherein:
• the pyranose oxidase is sequentially added to the sample after glucose oxidase is added to the sample;
• and
• the pyranose oxidase causes a second chemiluminescent reaction with the sample;
• (e) measuring photons from a second light response resulting from the second chemiluminescent reaction;
• and
• (f) calculating a ratio of 1,5-anhydroglucitol and the first analyte based on the measured photons from the first light response to the photons from the second light response, wherein:
• measuring photons from the first light response resulting from the first chemiluminescent reaction and measuring photons from the second light response resulting from the second chemiluminescent reaction comprises measuring photons with a light detector;
• and
• comparing the photons from the first light response to the photons from the second light response to calculate a ratio of 1,5-anhydroglucitol and the first analyte comprises transmitting data to a computer processor.