Systems and methods for in situ optimization of tunable light emitting diode sources

A system comprising:

• a tunable light emitting diode source having a plurality of tuning states corresponding to a plurality of illumination spectrums;
• an image sensor;
• and
• a computing device comprising:
• a processor;
• and
• a non-transitory processor-readable storage medium in operable communication with the processor, wherein the storage medium contains one or more programming instructions that, when executed, cause the processor to perform the following:
• receiving at least a first data set and a second data set from the image sensor, the first and second data sets being collected by the image sensor based on illumination of a sample using, wherein the first data set and the second data set are associated with a first tuning state associated with a first illumination spectrum and a second tuning state associated with a second illumination spectrum of the tunable light emitting diode source, respectively;
• generating a score image data set based on an optical computation applied to the first and second data sets;
• determining whether the score image data set satisfies a tolerance level;
• and
• generating a set of test data based on the score image data set when the score image data set satisfies the tolerance level.