US 11,306,337 B2 · Polypeptides having hydrolytic activity on 1-kestose in the presence of sucrose but lacking sucrase (invertase) activity, polynucleotides encoding same and methods of producting and using same in industrial sucrose production from 1-kestose

Abstract

An industrial process using kestose hydrolases to enable the use of sugar mixtures containing 1-kestose in the industrial production of sucrose (as crystallized sugar) by providing a method for the conversion of 1-kestose into sucrose and fructose in a sugar solution, containing kestose and more than 10 mM sucrose (3 g/L), and comprising the enzymatic hydrolysis (preferably using 1-FEH enzymes—EC 3.2.1.153) of 1-kestose. The process further provides a method of producing a polypeptide having 1-kestose hydrolase activity and a composition comprising the polypeptide.

Claims7

Dependent claims

A method of improving sucrose crystal recovery, the method comprising contacting a sugar solution comprising kestose and more than 100 mM sucrose (30 g/L) with an enzyme having kestose hydrolase activity,

wherein the enzyme having kestose hydrolase activity has an amino acid sequence selected from the group consisting of SEQ ID NO. 5 and SEQ ID NO. 7;
and wherein the sugar solution is derived from sugarcane, sugar beets, and combinations thereof.

Dependencies are inferred from the claim text.

Facts

Application

15/775,690Filed 11 Nov 2016 · us provisional application

Priority

12 Nov 2015

Published

US 2019/0276864 A112 Sep 2019

Granted

19 Apr 2022

Term ends

Not estimatedThe source records don’t support an estimate.

Classification8 codes

C12P 19/12
Preparation of compounds containing saccharide radicals: disaccharides
C12N 9/2402
Acting on glycosyl compounds: hydrolysing O- and S- glycosyl compounds
C12P 19/02
Preparation of compounds containing saccharide radicals: monosaccharides
C12P 19/14
Preparation of compounds containing saccharide radicals: produced by the action of a carbohydrase , e.g. by alpha-amylase , e.g. by cellulase,…

Polypeptides having hydrolytic activity on 1-kestose in the presence of sucrose but lacking sucrase (invertase) activity, polynucleotides encoding same and methods of producting and using same in industrial sucrose production from 1-kestose

Life of this patent

Polypeptides having hydrolytic activity on 1-kestose in the presence of sucrose but lacking sucrase (invertase) activity, polynucleotides encoding same and methods of producting and using same in industrial sucrose production from 1-kestose

Life of this patent

Abstract

Claims7