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US 11,306,330 B2Granted
Glucoamylase engineered yeast and fermentation methods
AssigneeCargill, Incorporated
InventorsChris Miller, Brian Rush, Brynne Stanton, Joshua Dunn
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AssigneeCargill, Incorporated
InventorsChris Miller, Brian Rush, Brynne Stanton, Joshua Dunn
Estimating the term…
The invention is directed to an engineered yeast including an exogenous nucleic acid encoding a glucoamylase comprising SEQ ID NO:1 and SEQ ID NO:4, or a variant thereof. The engineered yeast are able to provide glucoamylase into a fermentation media and cause degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. High titers of bioproduct (e.g., 70 g/kg of ethanol) can be achieved, along with low residual glucose levels. Further the yeast exhibit good growth and bioproduct product at temperatures of 32° C. or greater.
An engineered yeast comprising an exogenous nucleic acid encoding a glucoamylase comprising a sequence having 90% or greater sequence identity to SEQ ID NO:1, wherein the yeast is capable of producing ethanol at a rate of 1 g/L*h or greater during a fermentation process.
A fermentation method for producing a bioproduct, comprising:
Dependencies are inferred from the claim text.