Expression process
AssigneeFujifilm Diosynth Biotechnologies UK Limited
InventorsFay Louise Saunders, Anna Louise Dodds, Adeline Marie Geraldine Bayard, Bhupendra Vallabh Kara
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Abstract
A process for the production of a target polypeptide is provided. The process comprises expression of an expression vector for expressing a target polypeptide in a host cell, preferably a mammalian cell, the expression vector comprising an expression cassette comprising a polynucleotide encoding a recombinant polypeptide operably linked to a fibronectin secretion leader sequence; and recovering the target polypeptide.
Claims10
A process of producing a target polypeptide which comprises:
- (a) expressing an expression vector for expressing a target polypeptide in a Chinese hamster ovary (CHO) host cell, the expression vector comprising an expression cassette comprising a polynucleotide encoding a recombinant polypeptide operably linked to a fibronectin secretion leader sequence comprising SEQ ID NO:
- 2 or a sequence having 90% sequence identity to SEQ ID NO:
- 2 and retaining the ability to secrete the target polypeptide;
- and
- (b) recovering the target polypeptide.
A process of producing a target polypeptide which comprises:
- (a) transfection or transformation of a Chinese hamster ovary (CHO) host cell with an expression vector for expressing a target polypeptide in a host cell, the expression vector comprising an expression cassette comprising a polynucleotide encoding the target polypeptide operably linked to a fibronectin secretion leader sequence comprising SEQ ID NO:
- 2 or a sequence having 90% sequence identity to SEQ ID NO:
- 2 and retaining the ability 10 secrete the target polypeptide;
- (b) culturing the host cell under conditions which allow proliferation of the host cell and expression and secretion of the target polypeptide from the host cell;
- and
- (c) recovering the target polypeptide.
A process for the production of a target polypeptide, the process comprising culturing a Chinese hamster ovary cell (CHO) transfected with an expression vector comprising an expression cassette comprising a polynucleotide encoding the target polypeptide operably linked to a fibronectin secretion leader sequence comprising SEQ ID NO:
- 2 or a sequence having 90% sequence identity to SEQ ID NO:
- 2 and retaining the ability to secrete the target polypeptide.
Dependencies are inferred from the claim text.