Transcription factors that regulate nicotine biosynthesis in tobacco
AssigneeNorth Carolina State University
InventorsRongda Qu, Bingwu Wang
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Abstract
The present invention provides nucleic acids encoding transcription factors and methods of using these nucleic acids to modulate nicotine production in plants and to produce plants having modulated nicotine production.
Claims20
An isolated nucleic acid molecule comprising a cDNA sequence selected from the group consisting of:
- (a) the nucleotide sequence of SEQ ID NO:
- 7;
- (b) a nucleotide sequence that is at least 95% identical to the nucleotide sequence of SEQ ID NO:
- 7;
- and
- (c) a nucleotide sequence that encodes a polypeptide having the amino acid sequence of SEQ ID NO:
- 8.
A method of producing a transgenic Nicotiana tabacum plant having decreased alkaloid content, comprising:
- (a) introducing into a Nicotiana tabacum plant cell a nucleic acid construct comprising at least 21 consecutive nucleotides of a cDNA molecule comprising a nucleotide sequence selected from the group consisting of:
- (i) the nucleotide sequence of SEQ ID NO:
- 7;
- (ii) a nucleotide sequence that is at least 95% identical to the nucleotide sequence of SEQ ID NO:
- 7;
- and
- (iii) a nucleotide sequence that encodes a polypeptide having the amino acid sequence of SEQ ID NO:
- 8;
- wherein the consecutive nucleotides are in sense orientation, antisense orientation, or both;
- and
- (b) regenerating the transgenic Nicotiana tabacum plant cell to produce a transgenic Nicotiana tabacum plant comprising the nucleic acid construct.
A tobacco plant regenerated from a tobacco plant cell comprising a mutant allele of a gene encoding a transcription factor that modulates nicotine biosynthesis, wherein the tobacco plant cell is produced by a method comprising:
- (a) introducing into a population of tobacco plant cells a reagent for site-directed mutagenesis comprising an oligonucleotide having a targeting sequence with at least 95% sequence identity to SEQ ID NO:
- 7, or a complement thereof, thereby producing the tobacco plant cell comprising the mutant allele of the gene encoding the transcription factor that modulates nicotine biosynthesis;
- and
- (b) regenerating a tobacco plant from the tobacco plant cell of step (a),
- thereby resulting in a tobacco plant comprising tobacco plant cells comprising the mutant allele of the gene encoding the transcription factor that modulates nicotine biosynthesis.
Dependencies are inferred from the claim text.