Targeted transposition for use in epigenetic studies
AssigneeActive Motif, Inc.
InventorsMary Anne Jelinek, Brian Stanley Egan, Joseph Fernandez
Life of this patent
Estimating the term…
Abstract
Disclosed herein are compositions, methods and kits useful for epigenetic analysis based on the use of transposons that are targeted to specific regions of chromatin based on DNA-DNA interactions, protein-protein interactions, RNA-RNA interactions, and nucleic acid-protein interactions.
Claims26
A binding protein-transposome complex, comprising a binding protein that binds a target, wherein the binding protein comprises Protein A, Protein G, a Protein A fusion protein or a Protein G fusion protein, said binding protein stably attached to a stable transposase:transposon complex (“transposome”), wherein the target is a protein that binds a nucleic acid-associated protein or protein domain, a DNA-binding site, or a protein that binds either of these.
A binding protein-transposome complex, comprising a non- antibody protein, wherein the non-antibody protein comprises Protein A, Protein G, a Protein A fusion protein or a Protein G fusion protein, said protein stably attached to a stable transposase:transposon complex (“transposome”), wherein the non-antibody protein is further bound to a second protein, wherein the second protein binds a nucleic acid-associated protein or protein domain, or a DNA-binding site.
A composition comprising:
- (a) a primary antibody, wherein the primary antibody binds a nucleic acid-associated protein or protein domain, or a DNA-binding site;
- (b) a secondary antibody bound to the primary antibody;
- and
- (c) a binding protein-transposome complex, comprising a non-antibody protein bound to the secondary antibody, wherein the non-antibody protein comprises Protein A, Protein G, a Protein A fusion protein or a Protein G fusion protein, said non-antibody protein stably attached to a stable transposase:transposon complex (“transposome”).
Dependencies are inferred from the claim text.