Heat-resistant luciferase
AssigneesToyo B-Net Co., Ltd., Toyo Ink Sc Holdings Co., Ltd.
InventorsAyumi Hori, Yutaka Yamagishi
Life of this patent
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Abstract
A new thermostable luciferase of the following mutant luciferase (a) or (b): (a) a mutant of a wild-type luciferase comprising the amino acid sequence of SEQ ID NO: 1, wherein phenylalanine at position 292 and/or phenylalanine at position 294 in the amino acid sequence of SEQ ID NO: 1 is substituted with another amino acid; or (b) a mutant of a luciferase having 93% or more homology with the amino acid sequence of SEQ ID NO: 1, wherein in the amino acid sequence of the mutant, the amino acid at a site corresponding to position 292 and/or position 294 in the amino acid sequence of SEQ ID NO: 1 is substituted with another amino acid.
Claims5
A thermostable luciferase of the following (a) or (b):
- (a) a luciferase comprising the amino acid sequence of SEQ ID NO:
- 1, wherein phenylalanine at position 292 in the amino acid sequence of SEQ ID NO:
- 1 is substituted with glycine, alanine, valine, leucine, isoleucine, methionine, glutamine, glutamic acid, lysine, arginine, or histidine, or wherein phenylalanine at position 292 and phenylalanine at position 294 in the amino acid sequence of SEQ ID NO:
- 1 are substituted with glycine, alanine, valine, leucine, isoleucine, methionine, glutamine, glutamic acid, lysine, arginine, or histidine;
- or
- (b) a luciferase having 93% or more sequence identity to the amino acid sequence of SEQ ID NO:
- 1 with the exception of the amino acid corresponding to residue at position 292 has the substitution of glycine, alanine, valine, leucine, isoleucine, methionine, glutamine, glutamic acid, lysine, arginine, or histidine or with the exception of the amino acid corresponding to residue at position 292 and position 294 have the substitution of glycine, alanine, valine, leucine, isoleucine, methionine, glutamine, glutamic acid, lysine, arginine, or histidine,
- wherein said thermostable luciferase has increased thermostability as compared to a wild-type luciferase.
An N-terminal fragment of a thermostable luciferase of the following (a) or (b)
- (a) a luciferase comprising the amino acid sequence of SEQ ID NO:
- 1, wherein phenylalanine at position 292 in the amino acid sequence of SEQ ID NO:
- 1 is substituted with glycine, alanine, valine, leucine, isoleucine, methionine, glutamine, glutamic acid, lysine, arginine, or histidine, or wherein phenylalanine at position 292 and phenylalanine at position 294 in the amino acid sequence of SEQ ID NO:
- 1 are substituted with glycine, alanine, valine, leucine, isoleucine, methionine, glutamine, glutamic acid, lysine, arginine, or histidine;
- or
- (b) a luciferase having 93% or more sequence identity to the amino acid sequence of SEQ ID NO:
- 1 with the exception of the amino acid corresponding to residue at position 292 has the substitution of glycine, alanine, valine, leucine, isoleucine, methionine, glutamine, glutamic acid, lysine, arginine, or histidine or with the exception of the amino acid corresponding to residue at position 292 and position 294 have the substitution of glycine, alanine, valine, leucine, isoleucine, methionine, glutamine, glutamic acid, lysine, arginine, or histidine,
- wherein the N-terminal fragment comprises position 292 of the amino acid sequence of SEQ ID NO:
- 1, and wherein said thermostable luciferase has increased thermostability as compared to a wild-type luciferase.
Dependencies are inferred from the claim text.