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US 11,306,284 B2Granted
Method for mechanical and hydrodynamic microfluidic transfection
AssigneeIndee. Inc.
InventorRyan Pawell
Life of this patent
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Abstract
Methods for introducing exogenous material into a cell are provided, which include exposing the cell to a transient decrease in pressure in the presence of the exogenous material. Also provided are devices for performing the method of the invention.
Claims28
A method introducing an exogenous material into a cell, comprising:
- inducing a first condition around the cell where there is a first ambient pressure insufficient to lyse the cell;
- inducing a second condition around the cell where a second ambient pressure is less than the first ambient pressure;
- and
- inducing a third condition around the cell where a third ambient pressure is greater than the second ambient pressure, the third condition being induced while the cell is in an unsteady flow, which creates a permeabilized cell membrane, allowing the exogenous material to be introduced into the cell.
1 more element
A method for introducing exogenous material into a cell, comprising:
- exposing the cell to a first pressure change under unsteady flow conditions, where the extracellular pressure is different than the intracellular pressure, to temporarily permeabilise the cell membrane without the cell becoming lysed;
- and
- exposing the cell to a second pressure change under unsteady flow to introduce the exogenous material into the cell, the first pressure change being a negative pressure change, the second pressure change being a positive pressure change along with unsteady flow.
Dependencies are inferred from the claim text.