A culture method employing a culture device for producing a cultured organ comprising an integrated product of a substance to be cultured and multiple ducts, the culture device comprising:
- a sealed container for containing the substance to be cultured, and said sealed container having a sealable input port for charging the substance to be cultured;
- the multiple ducts arranged in the sealed container, and each of said ducts having a plurality of micropores formed on the outer peripheral surface;
- a culture medium-feeding device connected to at least one of the multiple ducts, and said culture medium-feeding device feeding or circulating the culture medium to the at least one of the multiple ducts so that the at least one of the multiple ducts forms a culture medium-feeding duct, the culture medium-feeding device comprising a culture medium circulation circuit for circulating the culture medium to the culture medium-feeding duct, which is connected to both ends of the culture medium-feeding duct, a pump for circulating the culture medium, which is arranged in the culture medium circulation circuit, and a control box for controlling the circulating culture medium, which is arranged in the culture medium circulation circuit;
- and
- an excretory device connected to at least one duct other than the culture medium-feeding duct among the multiple ducts, said excretory device exhausting waste product permeating into the at least one duct other than the culture medium-feeding duct from the substance to be cultured through the micropores of the at least one duct other than the culture medium-feeding duct to the outside of the sealed container so that the at least one duct other than the culture medium-feeding duct forms an excretory duct, the excretory device comprising a waste product-containing vessel arranged in the outside of the sealed container, and a sending duct for sending the waste product accumulating within the excretory duct to the waste product-containing vessel,
- wherein the culture device further comprises at least one partition wall which is arranged in the sealed container so as to divide an inner space of the sealed container into multiple cells and is formed with multiple small holes, the multiple ducts being inserted into some of the multiple small holes in said at least one partition wall so as to be held, and the remaining multiple small holes in said at least one partition wall allowing both of the substance to be cultured and the culture medium to migrate between respective cells,
- wherein a plurality of the culture medium-feeding ducts is provided such that each of the culture medium-feeding ducts is branched into a plurality of small diameter ducts in the sealed container, each of the small diameter ducts having the micropores formed on the outer peripheral surface, and the small diameter ducts of each of the culture medium-feeding ducts are respectively inserted into the multiple small holes in said at least one partition wall,
- wherein the excretory duct is branched into a plurality of small diameter ducts in the sealed container, each of the small diameter ducts having the micropores formed on the outer peripheral surface, and the small diameter ducts of the excretory duct are respectively inserted into the multiple small holes in said at least one partition wall,
- wherein each of the culture medium-feeding ducts is provided with the culture medium-feeding device, and the culture medium circulating in each of the plurality of culture medium-feeding ducts is the same or are different from the culture medium circulating in another of the plurality of culture medium-feeding ducts,
- wherein the culture medium-feeding ducts are made to reproduce three-dimensionally a structure approximated to blood vessels or blood and lymphatic vessels of an organ in an organism, the organ being an implantation target by the cultured organ, by configuring the following:
- the number, mean diameter of the micropores, and quality of material of the culture medium-feeding ducts;
- the number, diameter, way of branching, and arrangement of the small diameter ducts of each of the culture medium-feeding ducts;
- and arrangement of the multiple small holes for the small diameter ducts of each of the culture medium-feeding ducts in said at least one partition wall, and
- wherein the excretory duct is made to reproduce three-dimensionally a structure approximated to an excretory duct of the organ that is the implantation target by the cultured organ, by configuring the following:
- the number, mean diameter of the micropores, and quality of material of the excretory duct of the culture device;
- the number, diameter, way of branching, and arrangement of the small diameter ducts of the excretory duct and arrangement of the multiple small holes for the small diameter ducts of the excretory duct in said at least one partition wall, said culture method comprising:
- culturing three-dimensionally the substance to be cultured in the sealed container by feeding the culture medium to the sealed container via each of the culture medium-feeding ducts by a respective culture medium-feeding device and by exhausting the waste product of the substance to be cultured to the outside of the sealed container via the excretory duct and the excretory device, while holding the substance to be cultured in the sealed container;
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- after the culture is completed, taking out the cultured product of the substance to be cultured as an integrated product with the ducts from the sealed container by disassembling the sealed container.