Process for separation, isolation and characterization of steviol glycosides
AssigneeHeartland Consumer Products, LLC
InventorsSteven J. Catani, Juan Navia
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Abstract
A comprehensive process for the separation, isolation and characterization of a combination of two or more steviol glycosides from extract of Stevia rebaudiana plants and their use in sweetening compositions are disclosed. Combinations of two or more steviol glycosides from Stevia rebaudiana are characterized. The combinations of two or more steviol glycosides can be used as sweetness enhancers, flavor enhancers and sweeteners in foods, beverages, cosmetics and pharmaceuticals. A method for isolating combinations of two or more steviol glycosides is also disclosed.
Claims5
A process for production a steviol glycoside composition comprising:
- a) extracting dried Stevia leaves for 2 h at 95° C. to form a crude extract;
- b) fractionating the crude extract on a reverse-phase column, wherein fractionation is conducted using a gradient of water and ethanol, wherein said gradient is used starting from 1:0, moving to 29.8:70.2, moving to 23.4:76.6, moving to 0:1, and moving to 1:1 water to ethanol, where the ethanol is food grade ethanol;
- wherein, fractions containing rebaudioside D, N, M, and A are collected and the water and ethanol removed, to provide a steviol glycoside composition comprising 23% rebaudioside D, 21% rebaudiosides N and M, and 2% rebaudioside A;
- and wherein,
- rebaudiosides D, N, M, and A are obtained in the claimed percentages without the need for crystallization.
Dependencies are inferred from the claim text.