US 11,306,074 B2 · Chemical and method for analyzing DNA sequence by visualizing single-molecule DNA and use thereof

Abstract

The present disclosure relates to a composition for analysis of DNA sequences and a method for analysis of DNA sequences by using the same and, more particularly, to a composition comprising the compound represented by Chemical Formula 1 and a method for analysis of DNA sequences, the method comprising a step of treating a sample with the same. The compound represented by Chemical Formula 1 in which TAMRA is linked to polypyrrole specifically binds an A/T base pair (W) to fluoresce alone without causing DNA photocleavage. Therefore, the compound is useful for DNA analysis particularly at the single DNA molecule level.

Claims10

Dependent claims

A composition for analysis of DNA sequences, the composition comprising a compound represented by the following Chemical Formula 1:

wherein,
n is an integer of 2 to 10, m, o, and p are each independently an integer of 1 to 10, and
X is one selected from the group consisting of a fluorescent protein, a photoprotein, a color reaction-catalyst, biotin, a fluorescent substance, a luminescent substance, and a chemiluminescent substance.

Dependencies are inferred from the claim text.

Facts

Application

16/446,887Filed 20 Jun 2019

Priority

KR 10-2018-008625524 Jul 2018

Published

US 2020/0031810 A130 Jan 2020

Granted

19 Apr 2022

Term ends

Not estimatedThe source records don’t support an estimate.

Classification6 codes

C07D 405/14
Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having…:…
C12Q 1/6806
Involving nucleic acids: preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/6816
Hybridisation assays: characterised by the detection means
C12Q 2563/107
Nucleic acid detection characterized by the use of physical, structural and functional properties: fluorescence

Chemical and method for analyzing DNA sequence by visualizing single-molecule DNA and use thereof

Life of this patent

Chemical and method for analyzing DNA sequence by visualizing single-molecule DNA and use thereof

Life of this patent

Abstract

Claims10