Systems and methods for point of use evacuation of an array

A method of moving a fluid within a sample container, the method comprising:

• (a) providing the sample container comprising
• (i) a first reaction zone comprising a fluid reservoir having a fluid disposed therein,
• (ii) a second reaction zone, and
• (iii) a fluid channel extending between the first reaction zone and the second reaction zone, the fluid channel comprising an openable seal disposed between the first reaction zone and the second reaction zone,
• the second reaction zone comprising the array assembly, the array assembly comprising
• a plurality of wells configured in an array,
• a vacuum port and a vacuum channel fluidly connected to the vacuum port, and
• a plurality of channels such that each well in the array is fluidly connected to the fluid channel and to the vacuum port and vacuum channel;
• (b) with the openable seal in a closed state, and with the vacuum port and vacuum channel opened, drawing, through the vacuum port and vacuum channel, a vacuum on the array assembly such that air is evacuated from the plurality of wells and the plurality of channels;
• (c) after drawing the vacuum on the array assembly, sealing the vacuum port and/or vacuum channel such that the plurality of wells and the plurality of channels are maintained under the vacuum, thereby forming an evacuated array;
• and
• (d) after sealing the vacuum port and/or vacuum channel, opening the openable seal such that the fluid is drawn into the plurality of wells via the plurality of channels.