Vascular cast-based scaffolds and methods of making the same
AssigneeWake Forest University Health Sciences
InventorsJames Yoo, In Kap Ko, Jennifer Huling, Anthony Atala
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Abstract
The present application relates to biomimetic three-dimensional (3D) scaffolds, constructs and methods of making the same. The three-dimensional scaffold can include a sacrificial internal cast and a durable external scaffold material, wherein the durable external scaffold material comprises a biocompatible material which completely surrounds the sacrificial internal cast and wherein the sacrificial internal cast be removed to yield a branching 3D network of hollow, vessel-like tubes that substantially mimics a native tissue or organ.
Claims21
A method of creating a three-dimensional, hollow vascular scaffold comprising:
- obtaining a sample comprising native tissue with an internal vasculature;
- perfusing a sacrificial internal casting composition into the native tissue vasculature;
- solidifying the sacrificial internal casting composition within the native tissue vasculature;
- digesting the native tissue, thereby creating a sacrificial solid cast of the native tissue vasculature;
- coating the sacrificial solid cast of the native tissue vasculature with a durable biocompatible outer scaffold material that surrounds the sacrificial solid cast of the native tissue vasculature;
- removing the sacrificial solid cast, thereby forming a three-dimensional, hollow vascular scaffold with a branching 3D network of hollow vessel-like tubes that mimics the original vasculature of the native tissue;
- and
- seeding the scaffold with a population of endothelial or endothelial progenitor cells.
A three-dimensional, hollow scaffold having a branching 3D network of hollow vessel-like tubes that mimics the original vasculature of native tissue comprising:
- a durable external scaffold layer on a portion of vascular corrosion cast;
- wherein the durable external scaffold layer comprises a biocompatible material that promotes endothelial cell or endothelial progenitor cell seeding and is formed by perfusing a sacrificial internal casting composition into a tissue vasculature;
- solidifying the sacrificial internal casting composition within the native vasculature;
- digesting the native vasculature, thereby creating a solid cast of the native vasculature;
- coating the solid cast with a biocompatible outer casting material;
- and removing the sacrificial internal casting composition thus forming a three-dimensional, hollow scaffold having a branching 3D network of hollow vessel-like tubes that mimics the original vasculature of native tissue.
Dependencies are inferred from the claim text.