Back to results
US 11,305,019 B2Granted
Functionalized nucleic acid nanostructures for RNA delivery
OwnerIan Fleming, Xi Chun Zhouinventors
Life of this patent
Estimating the term…
Abstract
The present disclosure provides cell-penetrating nucleic acid nanostructures well suited as transfection reagents for the delivery of bioactive agents to cells both in vivo and in vitro for research, diagnostic, and/or therapeutic purposes.
Claims20
A nucleic acid nanostructure, comprising:
- a DNA or RNA scaffold;
- at least one single stranded nucleic acid staple strand complementary to the scaffold;
- at least one cell penetrating peptide attached to the at least one staple strand;
- and
- at least one therapeutic substance attached to the at least one staple strand;
- wherein the at least one cell penetrating peptide is positively charged;
- wherein the nanostructure includes non-conjugated overhangs and cell penetrating peptide-conjugated overhangs;
- wherein the at least one therapeutic agent is selected from the group:
- siRNA, miRNA, shRNA, asRNA, mRNA, crRNA, tracrRNA, and a RNA vaccine.
6 more elements
A method of treating a subject, the method comprising:
- providing a nucleic acid nanostructure having:
- a DNA or RNA scaffold;
- at least one single-stranded nucleic acid staple strand complementary to the scaffold;
- at least one positively charged cell penetrating peptide attached to the at least one staple strand;
- and
- at least one therapeutic substance attached to the at least one staple strand;
- wherein the nanostructure includes non-conjugated overhangs and cell penetrating peptide-conjugated overhangs;
- wherein the at least one therapeutic agent is selected from the group:
- siRNA, miRNA, shRNA, asRNA, mRNA, crRNA, tracrRNA, and a RNA vaccine;
- and
- administering a therapeutically effective amount of the nucleic acid nanostructure to a subj ect.
8 more elements
A nucleic acid nanostructure, comprising:
- a DNA or RNA scaffold;
- at least one single stranded nucleic acid staple strand complementary to the scaffold;
- at least one cell penetrating peptide attached to the at least one staple strand;
- and
- at least one therapeutic substance attached to the at least one staple strand;
- wherein the at least one cell penetrating peptide is positively charged;
- wherein the nanostructure includes non-conjugated overhangs and cell penetrating peptide-conjugated overhangs.
4 more elements
A method of treating a subject, the method comprising:
- providing a nucleic acid nanostructure having:
- a DNA or RNA scaffold;
- at least one single-stranded nucleic acid staple strand complementary to the scaffold;
- at least one positively charged cell penetrating peptide attached to the at least one staple strand;
- and
- at least one therapeutic substance attached to the at least one staple strand;
- wherein the nanostructure includes non-conjugated overhangs and cell penetrating peptide-conjugated overhangs;
- and
- administering a therapeutically effective amount of the nucleic acid nanostructure to a subj ect.
6 more elements
Dependencies are inferred from the claim text.