Back to results
US 11,305,015 B2Granted
Cytomegalovirus vectors eliciting T cells restricted by major histocompatibility complex E molecules
AssigneeOregon Health & Science University
InventorsLouis Picker, Scott Hansen, Klaus Frueh, Daniel Malouli, Jay Nelson, Jonah Sacha, Meaghan Hancock
Life of this patent
Estimating the term…
Abstract
CMV vectors that lack active UL128, UL130, UL146 and UL147 proteins that may also comprise one or more microRNA regulatory elements (MRE) that restrict expression of the CMV are provided. Immunization with CMV vectors having the described features allows selection of different CD8+ T cell responses—CD8+ T cells restricted by MHC-Ia, MHC-II, or by MHC-E.
Claims19
A cytomegalovirus (CMV) vector comprising:
- (a) a first nucleic acid sequence that encodes at least one heterologous antigen;
- and
- (b) a second nucleic acid sequence comprising a first heterologous microRNA recognition element (MRE) operably linked to a UL122 (IE2) or UL79 CMV gene, wherein the MRE is complementary to a microRNA that is expressed by a cell of endothelial lineage, wherein the microRNA is miR-126-3p, miR-130a, miR-210, miR-221/222, miR-378, miR-296, or miR-328.
A cytomegalovirus (CMV) vector comprising:
- (a) a first nucleic acid sequence that encodes at least one heterologous antigen;
- (b) a second nucleic acid sequence comprising a first heterologous microRNA recognition element (MRE) operably linked to a UL122 (IE2) or UL79 CMV gene, wherein the MRE is complementary to a microRNA that is expressed by a cell of endothelial lineage;
- and
- (c) a third nucleic acid sequence comprising a second heterologous MRE operably linked to a UL122 (IE2) or UL29-UL79 CMV gene, wherein the MRE is complementary to a microRNA that is expressed by a cell of myeloid lineage, wherein the first microRNA is miR-126-3p, miR-130a, miR-210, miR-221/222, miR-378, miR-296, or miR-328 and the second microRNA is miR-142-3p, miR-223, miR-27a, miR-652, miR-155, miR-146a, miR-132, miR-21, or miR-125.
1 more element
Dependencies are inferred from the claim text.