Multiple antigen presenting system (MAPS)-based Staphylococcus aureus vaccine, immunogenic composition, and uses thereof

An immunogenic composition comprising:

• at least one immunogenic polysaccharide,
• at least two S. aureus or polypeptide antigens, and
• at least one pair of affinity molecules,
• wherein the at least one pair of affinity molecules comprises:
• (i) a first affinity molecule comprising biotin, and
• (ii) a second affinity molecule comprising a biotin-binding protein,
• wherein in each pair of affinity molecules:
• the first affinity molecule is associated with the at least one immunogenic polysaccharide, and
• the second affinity molecule is associated with at least one of the S. aureus polypeptide antigens,
• wherein the first affinity molecule non-covalently associates with the second affinity molecule to link the S. aureus polypeptide antigens and the immunogenic polysaccharide;
• wherein the at least one immunogenic polysaccharide comprises a type 1 capsular polysaccharide of Streptococcus pneumoniae, a type 5 capsular polysaccharide of S. aureus, and/or a type 8 capsular polysaccharide of S. aureus;
• and
• wherein the at least two S. aureus polypeptide antigens comprise (i) a hemolysin having the amino acid sequence of SEQ ID NO:
• 16;
• and (ii) a serine-aspartate repeat protein D (SdrD) protein having the amino acid sequence of SEQ ID NO:
• 7.