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US 11,304,982 B2Granted
Methods and compositions for modulating angiogenesis and vasculogenesis
AssigneeAtriCure, Inc.
InventorsMonique Dao, Ciara Tate, Casey C. Case
Life of this patent
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Abstract
Disclosed herein are methods and compositions for stimulating angiogenesis, using cells descended from marrow adherent stromal cells that have been transfected with sequences encoding a Notch intracellular domain. Applications of these methods and compositions include treatment of ischemic disorders such as stroke.
Claims15
A method for making a composition comprising angiogenin, angiopoietin-2 (ANG-2) and heparin-binding epidermal growth factor-like growth factor (HB-EGF), the method comprising
- (a) providing a culture of mesenchymal stem cells (MSCs),
- (b) contacting the cell culture of step (a) with a polynucleotide comprising sequences encoding a Notch intracellular domain (NICD) wherein said polynucleotide does not encode a full-length Notch protein,
- (c) selecting cells that comprise the polynucleotide of step (b), and
- (d) further culturing the selected cells of step (c) in the absence of selection and in the absence of trophic factors.
1 more element
Dependencies are inferred from the claim text.