Botulinum toxin and colloidal silver particles

A method of treating wrinkles, comprising

• administering a composition by injection to a subject in need thereof, the composition comprising botulinum toxin (BTX), colloidal silver particles, and at least one of water, saline and buffer, the composition comprising from 10±5 ppm to 30±5 ppm colloidal silver particles for every 100 units of BTX suspended in the water, and wherein the colloidal silver particles are in the form of a stable and colorless colloidal suspension of silver particles having an interior of metallic silver and an exterior surface of ionic silver oxide, wherein at least 75% of the silver particles have diameters between 0.005 micrometers and 0.015 micrometers, and
• storing the composition for a period of from 2 weeks to 4 months after preparation of the composition, prior to the administering of the composition to the subject;
• and
• wherein the administration is effective to treat static or functional wrinkles for a period of 7.5 months to 10 months.