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US 11,304,440 B2Granted
Tobacco having altered leaf properties and methods of making and using
AssigneeAltria Client Services LLC
InventorsChengalrayan Kudithipudi, Alec J. Hayes, Marcos Fernando de Godoy Lusso, Jerry Whit Morris
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Abstract
This disclosure provides tobacco plants containing a PMT RNAi and tobacco plants having a mutation in PMT, and methods of making and using such plants.
Claims20
A method of generating a transgenic Nicotiana tabacum plant comprising:
- (a) introducing an RNAi nucleic acid molecule to a N. tabacum cell, wherein said RNAi nucleic acid molecule comprises a first nucleic acid sequence at least 95% identical to SEQ ID NO:
- 11, and a second nucleic acid sequence at least 95% identical to SEQ ID NO:
- 12, and a spacer nucleic acid comprising a sequence at least 95% identical to SEQ ID NO:
- 13 between said first nucleic acid sequence and said second nucleic acid sequence;
- (b) generating at least one transgenic N. tabacum plant from said N. tabacum cell;
- and
- (c) selecting at least one transgenic N. tabacum plant generated in step (b) comprising said RNAi construct, wherein said at least one transgenic N. tabacum plant comprises a reduced amount of nicotine as compared to a control plant lacking said RNAi nucleic acid molecule.
4 more elements
Cured tobacco material comprising leaf from a Nicotiana tabacum plant comprising an RNAi nucleic acid molecule, wherein said RNAi nucleic acid molecule comprises a first nucleic acid sequence at least 95% identical to SEQ ID NO:
- 11, and a second nucleic acid sequence at least 95% identical to SEQ ID NO:
- 12, and a spacer nucleic acid comprising a sequence at least 95% identical to SEQ ID NO:
- 13 between said first nucleic acid sequence and said second nucleic acid sequence, and wherein said cured tobacco material comprises a reduced amount of nicotine as compared to cured tobacco material from a control plant lacking said RNAi nucleic acid molecule.
Dependencies are inferred from the claim text.