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US 11,304,425 B2Granted
Glycosylated beta-galactosidase compositions having improved transgalactosylating activity
AssigneeNovozymes A/S
InventorJeppe Wegener Tams
Life of this patent
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Abstract
The present invention relates to compositions, particularly liquid compositions, comprising polypeptides having beta-galactosidase activity, methods of making said compositions, and uses of the compositions for making e.g. dairy products. The polypeptides having beta-galactosidase activity are modified by glycation of lysine and/or arginine residues by incubating the enzyme in the presence of reducing sugars, optionally combined with a heat treatment. Thereby, transgalactosylating activity is increased.
Claims35
A formulation comprising:
- (a) 30-90 wt. % of a reducing sugar, wherein the reducing sugar is fructose, galactose, glucose, glyceraldehyde, ribose, xylose, cellobiose, maltose, or lactose, and
- (b) a polypeptide of glycoside hydrolase family 2 and enzyme class EC 3.2.1.23 or 3.2.1.108 comprising at least one lysine and/or arginine residue which is glycated by the reducing sugar, wherein the polypeptide has beta-galactosidase activity and transgalactosylating activity.
Dependencies are inferred from the claim text.