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US 11,300,559 B2Granted
Vitamin B2 detection by mass spectrometry
AssigneeQuest Diagnostics Investments Incorporated
InventorsChangming Yang, Sum Chan
Life of this patent
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Abstract
Methods are described for measuring the amount of a vitamin B2 in a sample. More specifically, mass spectrometric methods are described for detecting and quantifying vitamin B2 in a sample utilizing on-line extraction methods coupled with tandem mass spectrometric techniques.
Claims7
A method for determining the amount of vitamin B2 in a biological sample from a human, said method comprising:
- (a) adding internal standard to the sample;
- (b) purifying the sample and the internal standard by high performance liquid chromatography (HPLC);
- (c) ionizing vitamin B2 and the internal standard by electrospray ionization (ESI) in positive ion mode to produce one or more ions detectable by tandem mass spectrometry;
- (d) determining the amount of said one or more ions by mass spectrometry, wherein a fragment ion of the internal standard comprises a mass to charge ratio of 246.20±0.50;
- and
- (e) determining the amount of vitamin B2 in the sample from the amount of said one or more ions of vitamin B2 in step (c).
3 more elements
Dependencies are inferred from the claim text.