Method for designing primer, primer, primer set, DNA amplification method, and analysis method

Abstract

A method for designing a primer supplied to a PCR reaction includes a primer designing step of defining a 5′-end-side base sequence of the primer so as to include a base sequence or its complementary base sequence, the base sequence enabling identification of, among thermal cycles in the PCR reaction, a synthesis cycle of a DNA molecule in an amplification product obtained by the PCR reaction performed with the primer, the identification being based on base-sequence data obtained by sequencing the amplification product.

Dependencies are inferred from the claim text.