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US 11,299,771 B2Granted
Enhanced DNA sensing via catalytic aggregation of gold nanoparticles by DNA hybridization chain reaction
AssigneeBoise State University
InventorsJeunghoon Lee, Bernard Yurke, Elton Graugnard, Will Hughes, Bert Huttanus
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Abstract
The present invention provides compositions and methods for colorimetric detection schemes for detecting a variety of biomolecules. The compositions and methods employ DNA hybridization chain reaction for catalytic aggregation of gold nanoparticles. In this catalytic aggregation scheme, a single target DNA strand triggers the formation of multiple inter-particle linkages in contrast to the single linkage formed in conventional direct aggregation schemes.
Claims4
A kit comprising:
- a first gold nanoparticle comprising a first linker sequence which will hybridize to a contiguous target sequence a′ and b′, wherein said first linker sequence has a thiol group on the 3′ or 5′ end, and comprises:
- (i) a sequence a which is the complement of said target sequence a′, (ii) sequence b which is the complement of said target sequence b′, (iii) a b′ sequence, and (iv) a hairpin loop with sequence c which is formed by hybridization of sequences b and b′ in absence of said target sequence a′ and b′;
- and
- a second gold nanoparticle comprising a second linker sequence, wherein said second linker sequence will hybridize to a contiguous target sequence a′ and b′ and comprises (i) the complement of said target sequence b′, (ii) a less than full length complement of said target sequence a′, and (iii) the complement of said hairpin loop with sequence c, and does not form a hairpin in the presence or absence of the target sequence, and
- optionally, an RNA extractant buffer, a reaction buffer, a negative control sample, a positive control sample, a reaction container, a colorimetric chart, a packaging material, at least one biological sample preservative or additive, or a combination thereof;
- and
- instructions for use in detecting said target sequence.
4 more elements
A gold nanoparticle aggregate comprising:
- (a) a target sequence with continuous sequences a′ and b′;
- (b) a gold nanoparticle comprising a spacer group and a first linker, wherein said first linker has a thiol group on the 3′ or 5′ end and is capable of hybridizing to said target, said first linker comprising:
- i) sequence a which is the complement of target sequence a′,
- ii) sequence b which is the complement of target sequence b′,
- iii) a b′ sequence, and
- iv) a hairpin loop with sequence c which is formed by hybridization of sequences b and b′ in absence of said target;
- and
- (c) a second linker comprising a complement of the first linker and a complement of said target, wherein in the presence of said target, said first linker aggregates with said second linker, and wherein said second linker does not form a hairpin in the presence or absence of said target.
5 more elements
Dependencies are inferred from the claim text.