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US 11,299,770 B2Granted
On-slide staining by primer extension
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsNikolay Samusik, Garry P. Nolan, Yury Goltsev
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Abstract
A method for analyzing planar sample is provided. In some cases the method comprises: (a) incubating the planar sample with a capture agent that is linked to an oligonucleotide, wherein the capture agent specifically binds to complementary sites in the planar sample; (b) reading a fluorescent signal caused by extension of a primer that is hybridized to the oligonucleotide, using fluorescence microscopy. Several implementations of the method, and multiplexed versions of the same, are also provided.
Claims20
A composition comprising:
- (a) a biological sample;
- and
- (b) a plurality of antibodies;
- wherein:
- i. the plurality of antibodies are linked to single-stranded oligonucleotides, and
- ii. the plurality of antibodies are chemically cross-linked directly to the biological sample.
3 more elements
Dependencies are inferred from the claim text.