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US 11,299,769 B2Granted
Target reporter constructs and uses thereof
AssigneeRedVault Biosciences, LP
InventorsMichael L. Metzker, Christopher August Weier
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Abstract
Provided herein are methods and compositions for the detection of target nucleic acids using target reporter constructs (TRCs) which comprise target sequences complementary to the target nucleic acid. Further provided are methods of replicating the TRCs using rolling circle replication and/or rolling circle amplification to produce replicated TRCs which can be detected using probe sequences within the replicated TRCs.
Claims20
A target reporter construct (TRC) for detecting target nucleic acids, the TRC being a closed, partially single-stranded nucleic acid comprising:
- (a) one or more target sequences complementary to the target nucleic acids, wherein the target sequences are toehold switches comprising a short duplex structure with an overhang;
- (b) a bridge sequence forming a double-stranded portion of the TRC, wherein the bridge sequence comprises one or more stabilizing sequences for the formation of nanospheres during replication;
- and
- (c) an accessory sequence comprising a multifunctional probe sequence.
1 more element
Dependencies are inferred from the claim text.