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US 11,299,767 B2Granted
Method for generating a three-dimensional nucleic acid containing matrix
AssigneePresident and Fellows of Harvard College
InventorsGeorge M. Church, Je-Hyuk Lee, Richard C. Terry, Evan R. Daugharthy
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Abstract
Methods of making a three-dimensional matrix of nucleic acids within a cell is provided.
Claims21
A method of analyzing a biological sample, comprising:
- (a) permeabilizing said biological sample, wherein said biological sample comprises a plurality of cells, wherein a cell of said plurality of cells comprises cellular nucleic acid molecules having a relative three-dimensional (3D) spatial orientation within said cell;
- (b) generating a 3D matrix comprising said cellular nucleic acid molecules attached thereto, wherein said 3D matrix preserves said relative 3D spatial orientation;
- (c) contacting said 3D matrix with reagents to selectively remove a non-nucleic acid component from said biological sample;
- and
- (d) using 3D fluorescence imaging to identify said cellular nucleic acid molecules and said relative 3D spatial orientation of said cellular nucleic acid molecules within said cell.
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Dependencies are inferred from the claim text.