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US 11,299,765 B2Granted
Methods and compositions for preparing sequencing libraries
AssigneeIllumina Cambridge Limited
InventorsRoberto Rigatti, Niall Anthony Gormley
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Abstract
Embodiments provided herein relate to methods and compositions for next generation sequencing. Some embodiments include the preparation of a template library from a target nucleic acid in contact with a surface, and sequencing the library on the surface.
Claims15
A method of preparing a population of target nucleic acids for sequencing comprising:
- (a) providing a substrate having a surface comprising capture probes;
- (b) contacting the surface with a reaction volume comprising a plurality of template nucleic acids and transposomes, wherein each transposome comprises a transposase and a transposon sequence comprising a sequence that hybridizes to the capture probes, wherein the template nucleic acids are prepared by contacting a target nucleic acid with the plurality of transposomes in the presence of the surface;
- (c) associating the template nucleic acids with the capture probes;
- and
- (d) sequencing the template nucleic acids on the surface.
2 more elements
Dependencies are inferred from the claim text.