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US 11,299,757 B2Granted
Mutant gene associated with improvement in ethanol productivity via ethanol fermentation and method for producing ethanol using the same
AssigneeToyota Jidosha Kabushiki Kaisha
InventorsJunji Ito, Toru Onishi, Nobuki Tada, Rie Hirao
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Abstract
This invention is intended to improve the ethanol fermentation ability of a yeast strain having xylose-metabolizing ability with the use of a mutant gene encoding a mutant protein comprising a consensus sequence comprising a substitution of amino acid in the 30th position in SEQ ID NO: 1, amino acid in the 43rd position in SEQ ID NO: 4, and amino acid in the 31st position in SEQ ID NO: 7 with other amino acid residues.
Claims4
A mutant gene encoding a mutant CDC123 (cell division cycle 123) protein, wherein said mutant CDC123 protein comprises:
- (a) the amino acid sequence of SEQ ID NO:
- 3;
- or
- (b) an amino acid sequence having 95% or higher identity to the amino acid sequence of SEQ ID NO:
- 3 in which an amino acid residue corresponding to the 324th position from the N terminus of the amino acid sequence of SEQ ID NO:
- 3 is cysteine.
3 more elements
Dependencies are inferred from the claim text.