US 11,299,749 B2 · Cell-specific expression of modRNA

Abstract

Disclosed is an expression regulatory system for cell-specific transcription (expression) of a protein of interest, for example a cell cycle inducer that reactivates proliferation in adult or neonatal cardiomyocytes or insulin-producing beta cells. The expression regulatory system comprises a first nucleic acid that encodes a microRNA recognition element that specifically binds a target cell miR, and a translation suppressor protein; and a second nucleic acid that comprises a suppressor protein interaction motif that binds the translation suppressor protein, and a gene that encodes a protein of interest. When a cell of interest is co-transfected with the first and second nucleic acids of the system, the protein of interest expressed in a cell-specific fashion.

Claims18

Dependent claims

An expression regulatory system for cell-specific expression of a gene of interest (GOI) comprising:

a first nucleic acid that encodes a microRNA (miR) recognition element near its 3′UTR that specifically binds a target cell-specific miR, and a translation suppressor protein;
and
a second nucleic acid that comprises a suppressor protein interaction motif that binds the translation suppressor protein, and a gene that encodes a protein of interest, wherein the protein of interest is a cell cycle inducer protein;
wherein
(a) said first nucleic acid comprises the nucleotide sequence of SEQ ID NO:
2, SEQ ID NO:
3, or SEQ ID NO:
4,
(b) said second nucleic acid comprises the nucleotide sequence of SEQ ID NO:
5, SEQ ID NO:
6, SEQ ID NO:
7 or SEQ ID NO:
8,
or both (a) and (b).

11 more elements

Dependencies are inferred from the claim text.

Cell-specific expression of modRNA

Life of this patent

Cell-specific expression of modRNA

Life of this patent

Abstract

Claims18