Manipulation of genes involved in signal transduction to control fungal morphology during fermentation and production
AssigneeZymergen Inc.
InventorsKenneth S. Bruno, Sachin Jain, Brandon Pfannenstiel, Edyta Szewczyk
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Abstract
The present disclosure provides a microbial genomic engineering method and system for transforming, screening, and selecting filamentous fungal cells that have altered morphology and/or growth under specific growth conditions. The method and system utilize high-throughput (HTP) methods to produce filamentous fungal production strains with a desired morphological phenotype.
Claims29
An engineered Aspergillus strain capable of responding to osmotic stress substantial similar to a parental strain of Aspergillus from which the engineered Aspergillus strain is generated, wherein the engineered Aspergillus strain comprises a heterologous modification of an Aspergillus niger (A. niger) orthologue of a Saccharomyces cerevisiae (S. cerevisiae) sln1 gene that produces a reduced amount and/or less active form of the polypeptide encoded by the A. niger orthologue of the S. cerevisiae sln1 gene in the engineered Aspergillus strain as compared to cells of the parental Aspergillus strain.
An engineered Aspergillus strain comprising a heterologous modification of a nikA gene that produces a reduced amount and/or less active form of a polypeptide encoded by the nikA gene in the engineered Aspergillus strain as compared to cells of die parental Aspergillus strain, wherein the heterologous modification comprises replacement of the nikA gene of the parental Aspergillus strain with a single nucleotide polymorphism (SNP) containing version of the nikA gene, wherein the SNP containing version of the nikA gene comprises a nucleotide substitution at position 814 of the nikA gene of the parental Aspergillus strain.
Dependencies are inferred from the claim text.