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AssigneeThe Rockefeller University
InventorsLuciano Marraffini, Gregory Goldberg, Poulami Samai
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Provided are compositions and methods for cleaving a DNA sequence in a cell. The methods involve comprising introducing into a cell a recombinant vector containing a clustered regularly interspaced short palindromic repeats (CRISPR) system. The system includes a CRISPR RNA (crRNA) targeted to a DNA sequence in the cell that is operatively linked to a promoter; and CRISPR-associated enzymes (Cas) 10, Cas6, and at least one Csm protein. The Cas 10 cleaves the DNA sequence only during transcription of the DNA sequence that is operatively linked to the promoter. Also provided are recombinant vectors for modifying cells, cells that contain the recombinant vectors and modifications introduced by them, and kits that include the modified vectors.
A method for transcription-restricted DNA modification that modifies a DNA sequence in a chromosome of a cell, the method comprising modifying the DNA sequence in the chromosome of the cell only during transcription of said DNA sequence, and wherein the modifying comprises cutting only a non-template strand only within said DNA sequence, by expressing in the cell from a recombinant vector a clustered regularly interspaced short palindromic repeats (CRISPR) system, the CRISPR system comprising nucleotide sequences encoding:
Dependencies are inferred from the claim text.