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AssigneeStratos Genomics, Inc.
InventorsMark Stamatios Kokoris, Marc Prindle, Melud Nabavi, Craig Ostrander, Taylor Lehmann, Samantha Vellucci, Michael Kovarik, Jack Chase, Robert Busam, Miranda Lahman
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Recombinant DPO4-type DNA polymerase variants with amino acid substitutions that confer modified properties upon the polymerase for improved single molecule sequencing applications are provided. Such properties may include enhanced binding and incorporation of bulky nucleotide analog substrates into daughter strands and the like. Also provided are compositions comprising such DPO4 variants and nucleotide analogs, as well as nucleic acids which encode the polymerases with the aforementioned phenotypes.
An isolated recombinant DNA polymerase, which recombinant DNA polymerase comprises an amino acid sequence that is at least 90% identical to SEQ ID NO:1, which recombinant polymerase comprises mutations at amino acid positions 76, 78, 79, 82, 83, and 86, wherein the identification of positions is relative to wildtype DPO4 polymerase (SEQ ID NO:1), and which recombinant DNA polymerase exhibits DNA polymerase activity.
Dependencies are inferred from the claim text.