Modified lipase and use thereof
AssigneeAmano Enzyme Inc.
InventorsKazunori Yoshida, Tetsuya Takahashi, Kazuhiko Ishikawa
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Abstract
The present invention has an object of providing a modified lipase having excellent stability and reactivity at a high temperature. Provided is a modified lipase with improved reactivity and/or stability at a high temperature, the modified lipase having an amino acid sequence including one or more amino acid substitutions which are selected from the group consisting of T130C-S153C, A249P, F259Y, S282P, S283Y and S300P in the amino acid sequence represented by SEQ ID NO: 1, or an amino acid sequence having 90% or more sequence identity therewith.
Claims13
A modified lipase with improved reactivity and/or stability at a high temperature, as compared to a lipase consisting of the amino acid sequence of SEQ ID NO:
- 1, the modified lipase having the amino acid sequence of SEQ ID NO:
- 1 except as having one or more amino acid substitutions selected from the group consisting of T130C-S153C, A249P, F259Y, 5282P, 5283Y, and S300P,
- or alternatively the modified lipase having an amino acid sequence having 92% or more sequence identity with the amino acid sequence of SEQ ID NO:
- 1 with one or more amino acid substitutions selected from the group consisting of T130C-S153C, A249P, F259Y, S282P, S283Y, and S300P, with the proviso that the amino acid sequence of the modified lipase is different from the amino acid sequence of SEQ ID NO:
- 1 at a position or positions other than the position(s) at which the amino acid substitution(s) is(are) made;
- wherein the high temperature is a temperature above 40 Celsius degree.
A lipase with improved reactivity and/or stability at a high temperature, as compared to a lipase consisting of the amino acid sequence of SEQ ID NO:
- 1, comprising the amino acid sequence of any of SEQ ID NOs:
- 2 to 7, or an amino acid sequence having 92% or more sequence identity with the amino acid sequence of any of SEQ ID Nos:
- 2 to 7, with the proviso that the amino acid sequence is different at a position or positions other than those corresponding to cysteine at positions 130 and 153 in the amino acid sequence of SEQ ID NO:
- 2, proline at position 249 in the amino acid sequence of SEQ ID NO:
- 3, tyrosine at position 259 in the amino acid sequence of SEQ ID NO:
- 4, proline at position 282 in the amino acid sequence of SEQ ID NO:
- 5, tyrosine at position 283 in the amino acid sequence of SEQ ID NO:
- 6, and proline at position 300 in the amino acid sequence of SEQ ID NO:
- 7, when the respective amino acid sequences are used as a reference sequence to determine the sequence identity;
- wherein the high temperature is a temperature above 40 Celsius degree.
A modified lipase with improved reactivity and/or stability at a high temperature, as compared to a lipase consisting of the amino acid sequence of SEQ ID NO:
- 1, the modified lipase having the amino acid sequence of SEQ ID NO:
- 1 except as having the amino acid substitutions is T130C-S153C,
- or alternatively the modified lipase having an amino acid sequence having 90% or more sequence identity with the amino acid sequence of SEQ ID NO:
- 1 with one or more amino acid substitutions of T130C-S153C, with the proviso that the amino acid sequence of the modified lipase is different from the amino acid sequence of SEQ ID NO:
- 1 at a position or positions other than the position(s) at which the amino acid substitution is made;
- wherein the high temperature is a temperature above 40 Celsius degree.
Dependencies are inferred from the claim text.