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AssigneeGenomatica, Inc.
InventorsKevin Hoff, Cara Ann Tracewell, Kui Chan, Amit Shah, Joseph Warner, Michael Noble
Estimating the term…
The invention provides polypeptides and encoding nucleic acids of aldehyde dehydrogenase variants. The invention also provides cells expressing aldehyde dehydrogenase variants. The invention further provides methods for producing 3-hydroxybutyraldehyde (3-HBal) and/or 1,3-butanediol (1,3-BDO), or an ester or amide thereof, comprising culturing cells expressing an aldehyde dehydrogenase variant or using lysates of such cells. The invention additional provides methods for producing 4-hydroxybutyraldehyde (4-HBal) and/or 1,4-butanediol (1,4-BDO), or an ester or amide thereof, comprising culturing cells expressing an aldehyde dehydrogenase variant or using lysates of such cells.
An isolated nucleic acid molecule selected from:
An isolated polypeptide comprising an amino acid sequence referenced as any one of SEQ ID NO:1, 2, 3, or 7-123, wherein said amino acid sequence comprises one or more of the amino acid substitutions selected from C174S, M204R, C220V, C267A, C356T, R396H, E437P, F442Q, C464I, and A467V as compared to wild type aldehyde dehydrogenase of SEQ ID NO:1, 2, 3, or 7-123.
An isolated polypeptide comprising an amino acid sequence referenced as any one of SEQ ID NO:1, 2, 3, or 7-123, wherein said amino acid sequence comprises one or more of the amino acid substitutions selected from C174S, M204R, C220V, C267A, C356T, R396H, E437P, F442Q, C464I, and A467V as compared to wild type aldehyde dehydrogenase of SEQ ID NO:1, 2, 3 or 7-123, wherein said amino acid sequence, other than the one or more amino acid substitutions, has at least 80%, 85%, 90%, 95%, 98% or 99% sequence identity, or is identical, to an amino acids sequence referenced as SEQ ID NO:1, 2, 3, or 7-123.
Dependencies are inferred from the claim text.