Use of iNOS inhibitors to increase viral yield in culture
AssigneeGenzyme Corporation
InventorsPeter Pechan, Jeffery Ardinger, Abraham Scaria, Samuel Wadsworth
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Abstract
The use of iNOS inhibitors, including aurintricarboxylic acid, dexamethasone and valproic acid, to increase the yield of a variety of viruses in culture, including recombinant herpesviruses is described.
Claims9
A method for producing herpes simplex-1 virus (HSV-1) comprising culturing cells infected with said herpes simplex-1 virus in a cell culture medium that comprises aurintricarboxylic acid and serum, wherein the aurintricarboxylic acid is unfractionated, wherein aurintricarboxylic acid is at a concentration of about 5 μM to about 75 μM, and wherein the cells are 293 or Vero cells.
A method for producing HSV-1 d27.1 comprising:
- (a) infecting V27 cells with said HSV-1 d27.1;
- and
- (b) culturing said infected V27 cells in a cell culture medium comprising serum, and aurintricarboxylic acid, wherein aurintricarboxylic acid is at a concentration of about 5 μM to about 75 μM, and wherein aurintricarboxylic acid is unfractionated.
A cell culture comprising aurintricarboxylic acid, serum and 293 or Vero cells infected with HSV-1, wherein aurintricarboxylic acid is unfractionated, wherein aurintricarboxylic acid is at a concentration of about 5 μM to about 75 μM.
Dependencies are inferred from the claim text.