Engineered adult-like human heart tissue
AssigneeThe Trustees of Columbia University in the City of New York
InventorsGordana Vunjak-Novakovic, Keith Yeager, Kacey Ronaldson, Stephen Ma, Timothy Chen
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Abstract
A cardiac organoid containing 3-D matter of adult human heart tissue.
Claims27
An engineered three dimensional micro-tissue comprising a cell-hydrogel construct comprising electromechanically conditioned mature cardiomyocytes and dermal fibroblasts encapsulated in a fibrin hydrogel, and wherein the electromechanical conditioning comprises subjecting said construct to electrical stimulation that increases in intensity over a time period, wherein said electrical stimulation results in the micro-tissue comprising sarcomeres and a network of T-tubules.
An engineered three dimensional micro-tissue, comprising:
- a cell-hydrogel construct containing electromechanically conditioned dermal fibroblasts and mature cardiomyocytes, and wherein the electromechanical conditioning comprises subjecting said construct to electrical stimulation that increases in intensity over a time period, wherein said electrical stimulation results in the micro-tissue comprising a network of transverse-tubules, and further wherein the micro-tissue exhibits a positive force-frequency response.
An engineered three dimensional micro-tissue, comprising:
- a cell-hydrogel construct comprising electromechanically conditioned dermal fibroblasts and mature cardiomyocytes, wherein the electromechanically conditioning comprises stretching the construct between first and second pillars and subjecting said construct to electrical stimulation, said electrical stimulation including exposure to a frequency that gradually increase from 2 Hz to 6 Hz over a time period, and wherein said electrical stimulation results in the micro-tissue comprising sarcomeres and a network of transverse-tubules.
Dependencies are inferred from the claim text.