Microbial bioconversion of curcuminoids to calebin-A

A method for the bioconversion of curcuminoids to Calebin-A using fungal or bacterial species, said method comprising steps of:

• i. culturing the fungal or bacterial species in main two batches (flasks containing batch 1 and flasks containing batch 2) in 50 ml-500 ml volumes of suitable media and incubating at 35-37° C. with shaking at 100-120 rpm for 1 to 21 days;
• ii. on the 7th day of incubation, adding varying concentrations of curcuminoids to batch 1 flasks of step i) and incubating at 37° C. with 120 rpm shaking;
• iii. maintaining the batch 2 flasks of step i) without adding curcuminoids at 37° C. with 120 rpm shaking;
• iv. harvesting media from batch 1 and batch 2 flasks of step ii) and step iii) after 24, 48, 72, 96, 120 hrs;
• v. drying the harvested media of step iv) under vacuum and extracting using a suitable solvent;
• vi. identifying the presence of Calebin-A using HPLC (high performance liquid chromatography), LC-MS (liquid chromatography-mass spectrometry) and NMR (nuclear magnetic resonance);
• vii. refluxing the harvested media of step iv) with a suitable solvent followed by separating and drying the solvent layer under vacuum;
• and
• viii. identifying the presence of Calebin-A using HPTLC, HPLC, LC-MS and NMR, wherein the fungal species is Ovatospora brasiliensis MTCC 25236, and the bacterial species is Acinetobacter johnsonii NCIMB 9871 or Pseudomonas putida NCIMB 10007.