Gene combination capable of high-efficiency expression of recombinant human nerve growth factor
AssigneeXintrum Pharmaceuticals, Ltd.
InventorsHai Chen, Hongliang Sun, Yi Zhang, Yuesheng Wang
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Abstract
A gene combination capable of high-efficiency expression of rhNGF is provided to optimize gene expression regulation components of a recombinant human nerve growth factor. The gene combination enhances expression of rhNGF. As shown by experiments, the gene combination is capable of high-efficiency expression of a recombinant human nerve growth factor (rhNGF) with natural activity in a eukaryotic expression system.
Claims6
A human nerve growth factor pro gene nucleic acid molecule, comprising the nucleotide sequence SEQ ID NO:
- 7.
A nucleic acid molecule for expression of rhNGF,
- wherein the nucleic acid molecule comprises the nucleotide sequence SEQ ID NO:
- 7, operably linked to the nucleotide sequence of SEQ ID NO:
- 3 or SEQ ID NO:
- 5.
A method of producing rhNGF, comprising:
- producing rhNGF by expression of a nucleic acid molecule comprising a nucleotide sequence SEQ ID NO:
- 3 or SEQ ID NO:
- 5.
Dependencies are inferred from the claim text.