Purification of factor VIII subspecies
AssigneeTakeda Pharmaceutical Company Limited
InventorsMeinhard Hasslacher, Martin Feichtinger, Philipp M. Bärnthaler, Christa Mayer, Birgit Reipert, Mantas Malisauskas, Julia Anzengruber
Life of this patent
Estimating the term…
Abstract
The present invention relates to a method for purifying a Factor VIII (FVIII) subspecies from a composition comprising FVIII, said method comprising an anion exchange chromatography step, a size exclusion chromatography step, and a concentration step. The invention also relates to a composition comprising a purified FVIII subspecies.
Claims9
A method for purifying a Factor VIII (FVIII) subspecies from a composition comprising FVIII, said method comprising the steps of:
- (1) subjecting the composition comprising FVIII to anion exchange chromatography, and collecting the eluate comprising said FVIII subspecies;
- (2) subjecting the eluate of step (1) comprising said FVIII subspecies to size exclusion chromatography, and collecting the eluate comprising said FVIII subspecies;
- and
- (3) concentrating the eluate of step (2) comprising said FVIII subspecies,
- wherein said FVIII subspecies is a FVIII heavy chain that is associated with a FVIII light chain, and wherein the weight ratio of said FVIII subspecies in the composition is increased as compared to all other FVIII subspecies comprising a FVIII heavy chain that is associated with a FVIII light chain.
A method for purifying a Factor VIII (FVIII) subspecies from a composition comprising FVIII, said method comprising the steps of:
- (1) subjecting the composition comprising FVIII to anion exchange chromatography, and collecting the eluate comprising said FVIII subspecies;
- (2) subjecting the eluate of step (1) comprising said FVIII subspecies to hydrophobic interaction chromatography, and wherein the hydrophobic interaction chromatography is negative mode chromatography;
- and
- (3) concentrating the eluate of step (2) comprising said FVIII subspecies,
- wherein said FVIII subspecies is a FVIII heavy chain that is associated with a FVIII light chain, and wherein the weight ratio of said FVIII subspecies in the composition is increased as compared to all other FVIII subspecies comprising a FVIII heavy chain that is associated with a FVIII light chain.
A method for purifying a Factor VIII (FVIII) subspecies from a composition comprising FVIII, said method comprising the steps of:
- (1) subjecting the composition comprising FVIII to anion exchange chromatography, and collecting the eluate comprising said FVIII subspecies;
- and
- (2) concentrating the eluate of step (1) comprising said FVIII subspecies,
- wherein said FVIII subspecies is the FVIII 90 kDa heavy chain associated with a FVIII light chain and wherein the weight ratio of said FVIII subspecies in the composition is increased as compared to all other FVIII subspecies comprising a FVIII heavy chain that is associated with a FVIII light chain.
Dependencies are inferred from the claim text.