Modulation of rice MPG1 activity to increase biomass accumulation, grain yield, and stress tolerance in plants
AssigneeColorado State University Research Foundation
InventorsDaniel R. Bush, Amanda K. Broz, Bettina E. Broeckling, Michael M. Friedman
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Abstract
The invention provides methods for improving plant yield, biomass accumulation and stress tolerance. A novel AP2-like transcription factor MPG1, which when modulated impacts grain yield, biomass and abiotic and biotic stress tolerance when compared to non-modulated plants. The invention further provides methods using recombinant expression cassettes, host cells, transgenic plants and breeding methods using the same.
Claims9
A method of increasing biomass or seed yield in a plant comprising:
- introducing in said plant a polynucleotide encoding a MPG1 transcription factor polypeptide, wherein said MPG1 transcription factor polypeptide has MPG1 transcription factor protein activity and is a polypeptide comprising an amino acid sequence having at least 90% sequence identity to the sequence set forth in SEQ ID NO:2;
- and
- selecting for a plant with increased biomass or seed yield under non-stress conditions relative to a control plant without the polynucleotide encoding the MPG1 transcription factor polypeptide.
A method of producing a plant with increased biomass or seed yield, the method comprising:
- introducing in a plant cell a polynucleotide encoding a MPG1 transcription factor, wherein the MPG1 transcription factor comprises an amino acid sequence having at least 90% sequence identity to the sequence set forth in SEQ ID NO:2;
- regenerating a plant from the plant cell;
- and
- selecting for a plant with increased biomass or seed yield under non-stress conditions relative to a control plant without the polynucleotide encoding the MPG1 transcription factor polypeptide.
Dependencies are inferred from the claim text.