Method of producing proteins in filamentous fungi with decreased CLR2 activity

A method of producing a recombinant polypeptide in a filamentous fungus which is genetically modified by disruption of the clr2 gene to decrease or eliminate the activity of cellulase regulator 2 (CLR2) and disruption of the xyr1 gene to decrease or eliminate the activity of xylanase regulator 1 (XYR1) compared to the corresponding filamentous fungus not having the genetic modification which is cultured under the same conditions as the genetically modified filamentous fungus, wherein the filamentous fungus is Myceliophthora thermophila, wherein the filamentous fungus genetically modified to decrease or eliminate the activity of cellulase regulator 2 (CLR2) has been further genetically modified to express said recombinant polypeptide, wherein the recombinant polypeptide is expressed under the control of a promoter which is not activatable by CLR2, wherein the recombinant polypeptide is a polypeptide which is heterologous to the filamentous fungus, wherein the filamentous fungus genetically modified to decrease or eliminate the activity of cellulose regulator 2 (CLR2) has further been genetically modified by disruption of the alp1 gene to decrease or eliminate the activity of alkaline protease 1 (ALP1), said method comprising:

• (i) growing said genetically modified filamentous fungus in a culture medium which does not contain cellulose or any derivative thereof which is capable of inducing CLR2 activity;
• and
• (ii) isolating the recombinant polypeptide from the culture medium.