Compositions comprising a chimeric bacterial surface layer protein and methods of use thereof
AssigneeArizona Board of Regents on Behalf of the University of Arizona
InventorsGayatri Vedantam, Virinchipuram Viswanathan
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Abstract
The invention features engineered probiotic lacto acid bacteria (LAB) expressing a chimeric Clostridium difficile/Lactobacillus acidophilus SlpA, or fragment thereof, and its use for the treatment or prevention of Clostridium difficile infection and gut colonization.
Claims9
A chimeric nucleic acid molecule comprising a nucleic acid sequence comprising a phosphoglycerate mutase (pgm) constitutive promoter (pgm promoter) operatively linked to a nucleic acid sequence encoding a chimeric bacterial surface layer protein A (SlpA) comprising a Clostridium difficile variable domain having at least 85% identity to a Clostridium difficile variable domain having the sequence corresponding to SEQ ID NO:15 and a lactic acid bacterium (LAB) peptidoglycan anchor having at least 85% identity to a LAB peptidoglycan anchor having the sequence corresponding to SEQ ID NO:14.
Dependencies are inferred from the claim text.