Compounds for use in the elimination of pluripotent stem cells

A method of reducing the number or percentage of pluripotent stem cells or of enriching differentiating or differentiated cells in a cell population comprising pluripotent stem cells and differentiating cells or differentiated cells derived from the pluripotent stem cells, the method comprising the step of contacting the cell population with a compound according to the following general formula (1) and/or racemates, enantiomers, stereoisomers, solvates, hydrates or salts thereof:

• wherein:
• R1, R2 the same or independent from each other are hydrogen or represent a double bond from the nitrogen atom to the adjacent non-cyclic carbon atom;
• R3, R4 are the same or independently from each other selected from the group comprising hydrogen, linear or branched C1-C8-alkyl, C2-C8-alkenyl, C3-C8-cycloalkyl, C6-C10-aryl, C1-C8-alkoxy and halogen;
• E1, E2 are the same or independent from each other a 5- or 6-membered aromatic or heteroaromatic ring selected from the group comprising thiophenyl, pyrrolyl, pyridyl and phenyl;
• A is selected from the group of structural elements of formulas (2) and (3):
• —(Z)n—B—(Z)m— (2)
• —CHR′—(Z)p—, (3)
• wherein:
• B is selected from the group comprising C3-C8-cycloalkyl, C3-C8-heterocycloalkyl, C6-C10-aryl and C5-C10-heteroaryl;
• Z is the same or independently from each other selected from the group comprising —CH2—, —CHR′—, O, S, NH, NR′;
• R′ is the same or independently from each other selected from the group comprising hydrogen, C1-C8-alkyl, C2-C8-alkenyl, C3-C8-cycloalkyl and C6-C10-aryl;
• n, m are the same or independent from each other 0, 1 or 2;
• p is 1, 2, 3, 4, or 5;
• or contacting the cell population with a compound of formula (6)