Innervated artificial skin

A method of making a multi-layer silk composition comprising

• providing a first layer comprising silk fibroin and keratinocytes;
• providing a second layer comprising silk fibroin and fibroblasts;
• providing a third layer comprising silk fibroin and adipocytes;
• providing a plurality of nervous system cells;
• and
• associating the first layer, second layer, third layer, and plurality of nervous system cells to form a multi-layer silk composition, wherein at least some of the plurality of nervous system cells span at least two layers.

A method comprising

• a) providing a multi-layer silk composition comprising a first layer, a second layer, a third layer, and a plurality of nervous system cells, the first layer comprising silk fibroin and keratinocytes, the second layer comprising silk fibroin and fibroblasts, the third layer comprising silk fibroin and adipocytes, wherein at least some of the plurality of nervous system cells span at least two layers, wherein at least some of the cells in the composition are cancer cells;
• b) exposing the composition to one or more therapeutic agents and/or inflammatory agents;
• and
• c) characterizing the response of the cells in the composition to the one or more therapeutic agents.